Chapter 13

Data analysis tools

12 Click [Next].
The compensation process starts.
During acquisition, the auto loader analysis status
indicator LED blinks green and the rotor position
currently being acquired is displayed in the status
area.
When acquisition of all the sample tubes is finished,
the [Compensation Wizard] displays the
[Compensation Page] for the first channel as shown
in the screen on the right.

[Compensation Page] screen

[Channel]

This field displays the channel selected in the [Configuration Page].

[Workitem]

These fields are left blank.

[Adjust compensation and negative regions.]
[Plot 1]

The ungated dual-parameter density plot is displayed with FSC for the X-axis and
SSC for the Y-axis. You can change the scaling (except channels) for each X and
Y axis by right-clicking on the axis.
The plot contains a green 4-vertex polygon gate. You can move each vertex and
resize it as required to define the gating region. Any adjustment of the region
automatically updates the histogram plot on [Plot 2].

[Plot 2]

A single parameter plot (histogram) is displayed based on the Gate-1 region
defined on [Plot 1]. The X-axis on the plot is the current compensation parameter.
The plot contains a blue marker gate. You can move and resize the gate to specify
the positive population for that fluorochrome as Gate-2.

[Compensation Matrix]

The matrix displays the currently calculated spillover values.
After clicking [Next] in step 14, the values in the matrix are updated.

[Back]

Click to go back to the previous page if you need to change settings on the
previous page. Changes made to the current page are canceled.

[Next]

Click to save your changes and go to the next page.

[Cancel]

Click to cancel the compensation process.

13 Define the gate for each [Plot 1] and [Plot 2] to obtain spillover values of the
fluorochrome to the other detectors.

14 Click [Next] to proceed to the next compensation page.
The [Compensation Matrix] updates spillover values based on the gating defined on the previous pages.

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XF-1600 Instructions for Use