Chapter 13

Data analysis tools

⏺ Performing automatic compensation

1 Prepare each 200 µL of single-stained samples to be run for the
compensation, and place them in a rotor.
Regardless of the measurement conditions of a workitem or subset panel to which you want to apply spillover
values from this process, the aspiration volume set for this process is 200 µL. To ensure that the sample is
sufficient for the measurement, prepare more than the above required volume.

2 Select and activate the workitem or the subset panel to which you want to
apply spillover values from this compensation.

3 Click the [Compensation] tab in the ribbon menu.
4 Click [Auto] in the [Compensation] group.
The compensation wizard starts and the
[Configuration Page] is displayed.

[Configuration Page] screen

5 Select [Measure].
6 Select the parameter ([Area] or [Height]) you want to use for the
compensation.

7 Select the desired channels to automatically set up the compensation values.
8 Make sure that the analysis mode of the status area is [AutoLoader]. If the
mode is set to [Manual], press the mode switch.
The mode changes to [AutoLoader].

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XF-1600 Instructions for Use