Chapter 13

Data analysis tools

⏺ Performing manual compensation

1 Click the [Compensation] tab in the ribbon menu.
2 Click [Show Matrix] in the [Compensation] group.
The [Compensation Matrix] dialog box appears.

[Compensation Matrix] dialog box
The compensation matrix shows the spillover values from the last compensation. The rows are for each detector
and the columns are for each fluorochrome. For example, the spillover value of FL2_A (i.e. PE) into FL1_A (i.e.
FL1 detector) is 1.000 % in the above matrix with the condition of Area parameter.

3 Edit or reset spillover values.
Spillover grid control

Enter spillover values between -500.000 and 500.000. The names of the
rows and columns display parameters available. You can also check the
plots displayed in the workarea by scrolling the workarea.

[Parameter:]

Select [Area] or [Height] as the parameter displayed in the spillover grid
control.

[OK]

Click this button to save changes made in the matrix and close the dialog
box.

[Cancel]

Click this button to cancel any changes made in the matrix and close the
dialog box.

[Apply]

Click this button to apply changes made in the matrix. The dialog box does
not close.

4 Click [OK].

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XF-1600 Instructions for Use