Chapter 9

9.4

Cytometer QC and setup

Overview of daily QC

After the baseline is set up, the same lot of CyFlow DailyQC is used to run the daily QC to check for variations from
those baseline measurements to monitor the daily performance of the cytometer.
In this process, the software measures and records the %rCV (for all detectors) and the PMT voltages (for SSC and
all fluorescence detectors).
The results can also be viewed as radar charts and L-J charts.

9.4.1

Type of quality control

The L-J control is applied by the software to automatically track the cytometer setup values over time, monitor
performance, and detect shifts or trends in parameters as they occur.

9.4.2

When daily QC is performed

The following are guidelines for when daily QC should be performed:
• Daily before sample measurement
• After major troubleshooting or servicing of the instrument
• When you have concerns about the accuracy of the instrument's performance

Note:
If the baseline for the lot has not been determined, perform baseline setup before proceeding.

9.5

QC files

For monitoring the instrument's performance, a [QC File] is created for each QC bead lot and contains the following
information:
• Panel name
• Material name
• Lot number
• Expiration date
• Target/Limit information
• Baseline and daily QC results

Note:
Up to 40 QC files can be saved, and a maximum of 500 data points can be recorded for each QC item in
each QC file.
After 500 data points are reached, each new data point automatically deletes the oldest data point.

63/312
XF-1600 Instructions for Use