Chapter 9

9.2

Cytometer QC and setup

Overview of cytometer QC

Quality Control (QC) is a set of procedures to monitor the daily performance of the cytometer using CyFlow DailyQC
to ensure that the cytometer is working accurately and precisely.
The process involves:
• Automated baseline setup
• Daily QC
You first establish the cytometer's baseline by running CyFlow DailyQC, then run the same lot to perform daily QC.
The system monitors changes in the %rCV (percent robust coefficient of variation) and the PMT (photomultiplier
tube) voltages of daily QC results.
If a significant change in performance is indicated, please contact your authorized local Sysmex representative.

Information
Make sure that CyFlow DailyQC is used for both baseline setup and daily QC. If not used, proper baseline
setup/QC cannot be guaranteed.

Note:
Values of PMT voltages are used as reference.

9.3

Overview of baseline setup

The baseline setup workflow uses CyFlow DailyQC to define the cytometer's initial baseline status.
In this process, the %rCV for all detectors and the median SSC/fluorescence intensity are automatically measured.
The software algorithms use the information to determine cytometer settings and provide target values of %rCV for all
detectors and PMT voltages for SSC and all fluorescence detectors.

9.3.1 When baseline setup is performed
The following are guidelines for when baseline setup should be performed:
• No baseline has been previously set up
• When using a new QC bead lot for which the baseline has not yet been set up
• Daily QC has failed
For details, see the following:
(➤P.75 "9.9 Performing daily QC", step 9)
• After major troubleshooting or servicing of the instrument

62/312
XF-1600 Instructions for Use