Chapter 7

Instrument specifications

7.1.2 Performance
Item

Specification

Acquisition rate

50,000 events/second

Minimum detectable particle size

FSC: 0.5 μm, SSC: 0.5 μm

Fluorescence sensitivity

FITC < 100 MESF
PE < 50 MESF
APC < 100 MESF

Fluorescence linearity

FL1 R2 ≥0.97
FL2 R2 ≥0.97
FL6 R2 ≥0.97

Repeatability*

Intermediate

precision**

FSC and SSC sensitivity

CV (%) ≤10 %

Fluorescence sensitivity

CV (%) ≤10 %

WBC events

CV (%) ≤10 %

FSC and SSC sensitivity

CV (%) ≤10 %

Fluorescence sensitivity

CV (%) ≤10 %

WBC events
Carryover

CV (%) ≤10 %
<0.1 %

* Refers to the coefficient of variation when the measurement is repeated at least 10 times, using the following
samples and the measurement conditions (panels).
- For FSC/SSC and fluorescence sensitivity: CyFlow DailyQC by applying the panel [QC panel]
- For WBC events: Commercially available stabilized blood materials prepared at WBC 300-600/μL which are
qualified for use on the XF-1600, by applying the panel [WRP_R panel]
** Refers to the coefficient of variation when the measurement is repeated for 20 days by conducting 2 runs with
2 replicates each day in accordance with CLSI EP05-A3.
- For FSC/SSC and fluorescence sensitivity: CyFlow DailyQC by applying the panel [QC panel]
- For WBC events: Commercially available stabilized blood materials prepared at WBC 300-600/μL which are
qualified for use on the XF-1600, by applying the panel [WRP_R panel]

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XF-1600 Instructions for Use