Chapter 7

7.1.3

Instrument specifications

Optics

The semiconductor laser light (488 nm, 638 nm, and 405 nm) interrogates the cells and particles passing
through the flow cell. The forward scattered light is detected by a photodiode, and the side-scattered light and
fluorescence are detected by a photomultiplier. The light signals received by each detector are converted into
electrical signals, and by analyzing these signals, cell and particle information can be obtained.
Laser Diode (638 nm)

Laser Diode (405 nm)

Forward Scatter (FSC)

Flow cell

FL9

Laser Diode (488 nm)

FL6

FL10

FL7

FL8

Side Scatter (SSC)

FL5
FL2

FL4

Channel
FSC

Laser wavelength
488 nm

FL1

FL3

Fluorochromes or FSC/SSC

Optical filters

Forward scatter

-

SSC

Side scatter

-

FL1

FITC

530/30

FL2

PE

580/30

FL3

PE-DyLight 594

610/14

FL4

PerCP-Cy5.5

700/50

FL5

PE-Cy7

750LP

APC

660/20

FL7

Alexa Fluor 700

720/40

FL8

APC-Cy7

780/60

Pacific Blue

450/50

Pacific Orange

540/80

FL6

FL9
FL10

638 nm

405 nm

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XF-1600 Instructions for Use