Chapter 13

Data analysis tools

8 Click [Next].
The [Compensation Page] screen is displayed for
each selected channel.

[Compensation Page] screen
[Channel]

This field displays the first channel selected on the [Compensation Page].

[Workitem]

Each field displays the workitem information ([Primary ID], [Secondary ID], and
[Comment]) for the corresponding single-stained sample.

[Adjust compensation and negative regions.]
[Plot 1]

The ungated dual-parameter density plot is displayed with FSC for the X-axis and
SSC for the Y-axis. You can change the scaling (except channels) for each X and
Y axis by right-clicking on the axis.
The plot contains a green 4-vertex polygon gate. You can move each vertex and
resize it as required to define the gating region. Any adjustment of the region
automatically updates the histogram plot on [Plot 2].

[Plot 2]

A single parameter plot (histogram) is displayed based on the Gate-1 region
defined on [Plot 1]. The X-axis on the plot is the current compensation parameter.
The plot contains a blue marker gate. You can move and resize the gate to specify
the positive population for the fluorochrome as Gate-2.

[Compensation Matrix]

This matrix displays the currently calculated spillover values.
After clicking [Next] in step 10, the values in the matrix are updated.

[Back]

Click to go back to the previous page if you need to change settings on the
previous page. Changes made to the current page are canceled.

[Next]

Click to save your changes and go to the next channel.

[Cancel]

Click to cancel the compensation process.

9 Define the gate for each Plot 1 and Plot 2 to obtain spillover values of the
fluorochrome to the other detectors.

10 Click [Next] to proceed to the next compensation page.
The [Compensation Matrix] updates spillover values based on the gating defined in the previous pages.

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XF-1600 Instructions for Use