Chapter 12

Running samples

[Channel]

Displays all available channels. Select the checkbox for the desired
channels.
If you select the respective checkbox on the left of each channel name, all
of the 3 parameters ([Height], [Area], and [Width]) are enabled at once. If
you want to enable one or two of them, select the checkboxes for the
[Height], [Area], and [Width] columns, respectively. Data is acquired for the
channels with the blue or selected checkboxes. If the checkbox is gray, no
data is acquired for the channel. Click again to disable the selection.

[Name]

Set the name of each channel. You can enter up to 10 characters.
Duplicate channel names cannot be used.

[Height]

Select the checkbox to enable [Height] measurement for all the channels at once.
Click again to disable [Height] measurement for all the channels at once.

[Area]

Select the checkbox to enable [Area] measurement for all the channels at once.
Click again to disable [Area] measurement for all the channels at once.

[Width]

Select the checkbox to enable [Width] measurement for all the channels at once.
Click again to disable [Width] measurement for all the channels at once.

[Threshold]

This column defines the threshold value for events to be acquired.
Selecting the checkbox on the left includes the channel as the triggered
logical condition ([AND]). Enter a value from 1 to 2,500.

[Gain/PMT]

For FSC (Gain), enter a value from 0 to 2,560. For SSC and FL
(Photomultiplier Voltage), enter a value from 0 to 1,100.

[Flow Rate]

Click [High], [Middle], or [Low], and then select the desired flow rate from
the drop-down menu.
When [High] is selected:
• [90] (90 µL/min)
• [120] (120 µL/min)
When [Middle] is selected:
• [60] (60 μL/min)
• [30] (30 μL/min)
When [Low] is selected:
• [5] (5 µL/min)

[Volume]

Select the required sample volume.

[Required volume select]

The available options are [150], [250], [500], [1000], [1500], and [2000] μL.
By default, [250] μL is displayed.
• If a flow rate of [30] (30 μL/min) or [5] (5 μL/min) is selected, you cannot
select a volume between [500] and [2000] μL.
• When the selected volume is between [500] and [2000] μL, and the flow
rate has been changed to either [30] (30 μL/min) or [5] (5 μL/min), the
volume is automatically reset to [250] μL on the screen.
For more details on applicable flow rate and stop time, please see the
explanation for [Stop Time] in step 2 of "12.2.2 Setting stop conditions."
(➤P.164 "12.2.2 Setting stop conditions")

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XF-1600 Instructions for Use